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KMID : 0362420150530010009
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Journal of Korean Academy of Prosthodontics
2015 Volume.53 No. 1 p.9 ~ p.18
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The effect of blasting and anodizing-combined treatment of implant surface on response of osteoblast-like cell
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Seo Bo-Yong
Kim Young-Min
Choi Jae-Won
Yun Mi-Jung
Jeon You-Jin
Jeon Yun-Kyung
Kim Gyoo-Cheon
Huh Jung-Bo
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Abstract
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Purpose: The purpose of this study is to examine characteristics of implant surface with RBM and anodizing treatments, and to evaluate the responses of osteoblast-like cell (MG-63 cell).
Materials and methods: Grade IV titanium disks were fabricated (Diameter 10 mm, thickness 3 mm). Anodizing treatment (ASD) group, RBM and anodizing treatment (RBM/ASD) group, control (machined surface) group were divided. In this study, osteoblast-like cell was used for experiments. The experiments consist of surface characteristics evaluation by FE-SEM images, energy dispersive spectroscopy and stereo-SEM. In order to evaluate cell adhesion evaluation by crystal violet assay and observe cells form by confocal laser microscopy. To assess cell proliferation by XTT assay, cell differentiation by RT-PCR and mineralization by Alizarin red S stain assay. ELISA analyzer was used for Quantitative evaluation. Comparative analysis was run by one-way ANOVA (SPSS version 18.0). Differences were considered statistically significant at P<.05.
Results: In ASD group and RBM/ASD group, the surface shape of the crater was observed and components of oxygen and phosphate ions in comparison with the control group were detected. The surface average roughness was obtained 0.08 ± 0.04 ㎛ in the control group, 0.52 ± 0.14 ㎛ in ASD group and 1.45 ± 0.25 ㎛ in RBM/ASD group. In cell response experiments, ASD group and RBM/ASD group were significantly higher values than control group in cell adhesion and mineralization phase, control group was the highest values in the proliferative phase. In RT-PCR experiments, RBM/ASD group was showed higher ALP activity than other groups. RBM/ASD group in comparison with ASD group was significantly higher value for cell adhesion and proliferation phase.
Conclusion: In the limitation of this study, we are concluded that the surface treatment with RBM/ASD seems more effective than ASD alone or machined surface on cellular response.
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KEYWORD
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Anodizing treatment, RBM treatment, Surface roughness, Osteoblast-like cell, Adhesion, Proliferation, Differentiation
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